Hemoglobin of RBCs

HEMOGLOBIN (Hb) :-

Q1 What is name of most abundant protein in RBCs?

(a) Hemoglobin

(b) Myoglobin

(c) Actin

(d) Myosin

Q2 What is the molecular weight of Hemoglobin?

(a) 64,500 Da

(b) 65,450 Da

(c) 65,000 Da

(d) 64,00 Da

Q3 Nature of Hb is?

(a) Allosteric 

(b) Oligomeric 

(c) Conjugated 

(d) All of these 

Q4 How many polypeptide chains present in Hb?

(a) 4 

(b) 2 

(c) 1 

(d) 3

Q5 Polypeptide chains joined together by

(a) Non covalent bonds 

(b) Ionic bonds 

(c) Covalent bonds 

(d) Non of these

Q6 Hb present in RBCs of all except?

(a) Vertebrates 

(b) Invertebrates 

(c) Annelids 

(d) Many  Arthropods 

(e) Some Molluscs 

(f) Arctic fish 

(g) Option a b c d e

Q7 What is the name of polypeptide chains in Hb?

(a) Alpha 

(b) Theta 

(c) Beta

(d) Option a and c

Q8 Hb is a?

(a) Dimeric protein

(b) Trimeric Protein

(c) Tetrameric protein

(d) Option a b c 

Q9 What is the number of amino acid residues in one alpha chain?

(a) 240

(b) 141

(c) 140

(d) 145

Q10 What is the number of amino acids residues in one beta chain?

(a) 140

(b) 241

(c) 146

(d) 150

Q11 Which one is Adult Hb?

(a) HbA1

(b) HbF

(c) Xi2

(d) HbA2

Q12 Most abundant Hb in adult, HbA1 (98%) is consists of?

(a) 3alpha 2beta chains (3a2b) 

(b) 2a2b

(c) 1a3b

(d) 3a3b

A13 What polypeptide chains present in  HbA2 (2%) hemoglobin? 

(a) 2a2b

(b) 3a2b

(c) 2a2d

(d) 2s2b

NOTE :-

a = alpha

b= beta

d = delta

g = gama

z = zeta

ep = epsilon

Q14 What is the name of fetal Hb? 

(a) 2a2b

(b) 2a2xi

(c) 2a2g

(d) 2b3g

Q15 What is the name of Early Embryonic Hb?

(a) 2a2s

(b) 2a2b

(c) 2a2g

(d) 2z2ep

Q16 What is the time period of early embryonic Hemoglobin? 

(a) 2 - 4 week

(b) 4 - 8 week

(c) 6 - 8 week

(d) 8 - 10 week

Q17 Embryonic Hemoglobin is also known as?

(a) Fetal Hb

(b) Gower Hb

(c) HbA

(d) Option a and b both

Q18 What is the time period of early Fetal Hemoglobin?

(a) after 7 weeks

(b) after 8 weeks

(c) 6 - 8 week

(d) after 9 weeks

Q19 How many types of EHb? 

(a) 4

(b) 2

(c) 3

(d) 1

Q20 What type of hemoglobin are these - Hb Gower-1 (2z2ep), Hb Gower-2 (2a2ep) and Hb Gower-3 (2z2g)?

(a) HbF

(b) HbA

(c) HbE

(d) HbA2

Q21 Apart from O2 Hb also carry? 

(a) H+

(b) CO

(c) NO

(d) All of the above 

Q22 Is hemoglobin's affinity for NO is higher than O2? 

(a) True 

(b) False 

Q23 Hb can carry all of the following except? 

(a) CO

(b) O2

(c) O3

(d) CO2

Q24 Fetal Hb has higher affinity than HbA, ensuring adequate oxygen supply to fetus.

(a) True 

(b) False 

Q25 Embryonic Hemoglobin (HbE)  has low oxygen affinity, facilitating O2 transfer from mother to embryo. 

(a) True 

(b) False 

Q26 What are the functions of Hb? 

(a) Hb is a Transporter

(b) Role as a Buffer

(c) Control pH of blood

(d) Option b and c both

Q27 NO binds to which polypeptide chain of Hb?

(a) Gama chain 

(b) Alpha chain

(c) Zeta chain

(d) Beta chain

Q28 O2 cannot dissolved in water but dissolved in blood plasma.

(a) True

(b) False 

Q29 What carry most of the inhaled oxygen (98.5%) to tissues? 

(a) O2

(b) RBCs

(c) WBCs

(d) Hb

Q30 How many O2 molecules can bind with a Hb?

(a) 2

(b) 3

(c) 5

(d) 4

Q31 What is the protein part of Hb? 

(a) Heme

(b) RBCs

(c) Globin 

(d) Hemoglobin 

Q32 How many globin proteins are present in a Hb molecule?

(a) 1

(b) 5

(c) 3

(d) 4

Q33 How many Heme groups present in a Hb molecule?

(a) 4

(b) 3

(c) 5

(d) 2

Q34 What is the name of prosthetic group present in Hb? 

(a) Globin

(b) Myglobin

(c) Heme

(d) Oxygen 

Q35 What type of reaction occurs between Hb and O2? 

(a) Oxidation 

(b) Reduction 

(c) Oxygenation 

(d) Irreversible 

Q36 Hb and O2 reaction ia an irreversible Reaction.

(a) True 

(b) False 

Q37 Oxygen binds to Hb when Iron (Fe) in their

(a) Fe3+ state 

(b) Fe+ state

(c) Fe2+ state

(d) FeO state 

Q38 Fe3+ state doesn't bind O2 in Hb. 

(a) True

(b) False

Q39 Tense state of a Hb depicts as

(a) Oxyhemoglobin state

(b) Deoxyhemoglobin state

(c) Deoxygenation state

(d) Oxygenation state

Q40 Deoxy state of Hb is stabilized by 

(a) Covalent interactions

(b) ionic interactions 

(c) Non covalent interactions 

(d) Non ionic interactions

Q41 When one O2 - Hb it triggers a change in confrontation to R - state.

(a) True

(b) False

Q42 In relaxed state of Hb

(a) R - state  mean O2 -Hb bounded state

(b) O2 - Hb binding with much higher affinity

(c) Option a and b

(d) Non of above 

Q43 Last (4th) O2 -Hb binding affinity is much higher than first molecule

(a) True 

(b) False

OXYGEN - HEMOGLOBIN BINDING CURVE :-

Q44 Type of O2-Hb curve is

(a) Hyperbolic 

(b) Sigmoidal (S - shaped) 

(c) Bell shaped 

(d) exponential 

Q45 For hemoglobin this shape suggests that binding of oxygen at one site with Hb tetramer increases the likelyhood that O2 binds at remaining occupied sites.

(a) True

(b) False

Q46 O2 -Hb binding is a cooperative binding

(a) True

(b) False 

Q47 Unloading of one Oxygen at one heme facilitates the unloading of oxygen at the others, one by one.

(a) True

(b) False 

Q48 Binding reaction at each Hb molecule is

(a) Not independent of one another

(b) Dependent on one another 

(c) Independent of one another 

(d) Option a and b both

Q49 Its O2 -Hb increase affinity of IInd O2 -Hb affinity & oxygenation of IInd Hb increases affinity of IIIrd O2 -Hb so affinity for 4th  O2 -Hb = many times greater than that for the first. 

(a) True

(b) False 

Q50 Nitric oxide binds to the Proximal Histidine residue (HisF8) His 92 in human hemoglobin. 

(a) True

(b) False

ANSWER KEY:-

1 (a) 2 (b) 3(d) 4(a) 5(a) 6(f) 7(d) 8(c) 9(b) 10(c) 11(a) 12(b) 13(c) 14(c) 15(d) 16(c) 17(d) 18(c) 19(c) 20(c) 21(d) 22(a) 23(c) 24(a) 25(a) 26(d) 27(d) 28(b) 29(d) 30(d) 31(c) 32(d) 33(a) 34(c) 35(c) 36(b) 37(c) 38(a) 39(b) 40(b) 41(a) 42(c) 43(a) 44(b) 45(a) 46(a) 47(a) 48(d)  49(a)  50(a) 

🐥Next I'll post FUNCTIONAL DIFFERENCES BETWEEN Mb & Hb. 

Thank you so much for your interest in science.🤗🙏